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27301 Advanced Biochemistry

Danish title:

Videregående biokemi

Language:

Point( ECTS )

10

Course type:

MSc
Taught under open university
Generel competence course, MSc. Eng., Biotechnology
Technological specialization course, MSc. Eng., Food Technology

Schedule:

E2A (Mon 13-17) and E5B (Wed 13-17)

Location:

Campus Lyngby

Scope and form:

Lectures, group excercises, group work, project report and oral presentations

Duration of Course:

13 weeks

Date of examination:

E5B

Type of assessment:

Exam duration:

Aid:

Evaluation:

Not applicable together with:

Qualified Prerequisites:

,

General course objectives:

To give the students experience in project management, written and oral communication of scientific discoveries. To provide a deeper understanding of molecule structures and processes in the cell with focus on protein biochemistry, macromolecules metabolism, signal- and regulation mechanisms in the cell and in an organism. To give insight into bioanalytical techniques for identification, separation and characterisation of proteins. To relate biochemistry to biotechnology, cell physiology, immunology and physiology. To present theoretical and practical background for analytical and preparative biochemical methods.

Learning objectives:

A student who has met the objectives of the course will be able to:
  • Demonstrate the overall reactions in photosynthesis (C3- and C4-plants) and pentose phosphate pathway, regulations, comparisons to oxidative phosphorylations.
  • Describe glycogen metabolism.
  • Explain the role of membrane channels and pumps and describe signal transduction pathways; describe different membrane protein topologies and secondary structural elements in membrane spanning regions.
  • Describe metabolism of fatty acids, membrane lipids and steroids.
  • Describe mechanisms of post-translational processing and the influence of post-translational modifications on protein function.
  • Describe applications of bioanalytical techniques for identification, separation, and characterization of proteins. Outline “omics” techniques in systems biology and analysis of complex mixtures of proteins
  • Describe turnover of proteins, amino acid metabolism and biosynthesis of nucleotides and the enzymes involved in these reactions.
  • Construct integration of metabolism, interconnecting pathways, specific control sites, metabolic profile in muscle, brain, adipose tissue, kidneys, liver; metabolic changes after food intake and starvation.
  • Demonstrate detailed knowledge of how proteins are folded and their flexibility; prediction, engineering, determination and design of protein structures.
  • Demonstrate detailed knowledge of recombinant proteins, production, technology and validation.
  • Describe protein structures; determination of protein structures; protein interactions inclusive the relationship between structure and function.
  • To be able to, in a group, to define a focus area within biochemistry, which shall be described pedagogically and independently in a written review and which is presented by an oral presentation.

Content:

Protein biochemistry: Protein structure and ligand interactions. Protein engineering. Protein-protein interactions and regulation. Protein folding. Proteome analysis. Protein post-translational modifications. Recombinant proteins. Intracellular transport. Membrane transport. Membrane proteins.
Metabolism: Pentose-P-pathway. Photosynthetic production of energy and carbohydrates. Synthesis and oxidation of fatty acids. Protein synthesis. Protein metabolism and catabolism of amino acids. Synthesis of nucleotides. Synthesis of membrane lipids and steroids. Integration of metabolism.
Signal- and regulation mechanisms: Regulation of metabolism in the cell and in the organism. Hormones. Receptors and signal transduction.
Research methods: focus on proteins.

Green challenge participation:

Please contact the teacher for information on whether this course gives the student the opportunity to prepare a project that may participate in DTU´s Study Conference on sustainability, climate technology, and the environment (GRØN DYST). More infor http://www.groendyst.dtu.dk/english

Responsible:

Birte Svensson , Building 224, Ph. (+45) 4525 2740 , bis@bio.dtu.dk
Per Hägglund , Building 301, Ph. (+45) 4525 5503 , ph@bio.dtu.dk

Department:

27 Department of Systems Biology

Registration Sign up:

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Last updated: 04. maj, 2015