27301 Advanced Biochemistry
Point( ECTS )
Taught under open university
|Generel competence course, MSc. Eng., Biotechnology|
Technological specialization course, MSc. Eng., Food Technology
|E2A (Mon 13-17) and E5B (Wed 13-17)
Scope and form:
|Lectures, group excercises, group work, project report and oral
Duration of Course:
Type of assessment:
Not applicable together with:
General course objectives:
To give the students experience in project management, written and
oral communication of scientific discoveries. To provide a deeper
understanding of molecule structures and processes in the cell with
focus on protein biochemistry, macromolecules metabolism, signal-
and regulation mechanisms in the cell and in an organism. To give
insight into bioanalytical techniques for identification,
separation and characterisation of proteins. To relate biochemistry
to biotechnology, cell physiology, immunology and physiology. To
present theoretical and practical background for analytical and
preparative biochemical methods.
A student who has met the objectives of the course will be able to:
- Demonstrate the overall reactions in photosynthesis (C3- and
C4-plants) and pentose phosphate pathway, regulations, comparisons
to oxidative phosphorylations.
- Describe glycogen metabolism.
- Explain the role of membrane channels and pumps and describe
signal transduction pathways; describe different membrane protein
topologies and secondary structural elements in membrane spanning
- Describe metabolism of fatty acids, membrane lipids and
- Describe mechanisms of post-translational processing and the
influence of post-translational modifications on protein
- Describe applications of bioanalytical techniques for
identification, separation, and characterization of proteins.
Outline “omics” techniques in systems biology and analysis of
complex mixtures of proteins
- Describe turnover of proteins, amino acid metabolism and
biosynthesis of nucleotides and the enzymes involved in these
- Construct integration of metabolism, interconnecting pathways,
specific control sites, metabolic profile in muscle, brain, adipose
tissue, kidneys, liver; metabolic changes after food intake and
- Demonstrate detailed knowledge of how proteins are folded and
their flexibility; prediction, engineering, determination and
design of protein structures.
- Demonstrate detailed knowledge of recombinant proteins,
production, technology and validation.
- Describe protein structures; determination of protein
structures; protein interactions inclusive the relationship between
structure and function.
- To be able to, in a group, to define a focus area within
biochemistry, which shall be described pedagogically and
independently in a written review and which is presented by an oral
Protein biochemistry: Protein structure and ligand interactions.
Protein engineering. Protein-protein interactions and regulation.
Protein folding. Proteome analysis. Protein post-translational
modifications. Recombinant proteins. Intracellular transport.
Membrane transport. Membrane proteins.
Metabolism: Pentose-P-pathway. Photosynthetic production of energy
and carbohydrates. Synthesis and oxidation of fatty acids. Protein
synthesis. Protein metabolism and catabolism of amino acids.
Synthesis of nucleotides. Synthesis of membrane lipids and
steroids. Integration of metabolism.
Signal- and regulation mechanisms: Regulation of metabolism in the
cell and in the organism. Hormones. Receptors and signal
Research methods: focus on proteins.
Green challenge participation:
Please contact the teacher for information on whether this course
gives the student the opportunity to prepare a project that may
participate in DTU´s Study Conference on sustainability, climate
technology, and the environment (GRØN DYST). More infor
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Last updated: 04. maj, 2015